Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse

被引：9
|
作者：
Park, Chan Jin [1 ]
Chen, Guanglin [1 ]
Koo, Yongbum [2 ]
Lin, Po-Ching P. [1 ]
Cacioppo, Joseph A. [1 ]
Prohaska, Hailey [1 ]
Ko, CheMyong J. [1 ]
机构：
[1] Univ Illinois, Coll Vet Med, Comparat Biosci, 3806 VMBSB,MC-002,2001 South Lincoln Ave, Urbana, IL 61801 USA
[2] Inje Univ, Sch Biol Sci, Gimhae, South Korea
来源：
GENESIS | 2017年 / 55卷 / 12期
关键词：
BAC clone; estrogen receptor alpha; iCre recombinase; transgenic; ESTRADIOL-MEDIATED PROTECTION; EARLY POSTNATAL-DEVELOPMENT; GENE-EXPRESSION; BRAIN-INJURY; HYPOTHALAMIC NEURONS; REPRODUCTIVE-TRACT; TRANSGENIC MICE; ER-ALPHA; BETA; CORTEX;
D O I：
10.1002/dvg.23084
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and nonoverlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1-iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells. This novel transgenic mouse line will be a useful animal model for lineage-tracing Esr1-expressing cells, selective gene ablation in the Esr1-lineage cells and for generating global Esr1 knockout mice.
引用
收藏
页数：11
相关论文
共 50 条
  • [1] Generation of an estrogen receptor beta-iCre knock-in mouse
    Cacioppo, Joseph A.
    Koo, Yongbum
    Lin, Po-Ching Patrick
    Osmulski, Sarah A.
    Ko, Chunjoo D.
    Ko, CheMyong
    GENESIS, 2016, 54 (01) : 38 - 52
  • [2] Generation and Phenotypic Characterization of a Knock-in Mouse Model of McArdle Disease
    Lluis Andreu, Antoni
    Nogales-Gadea, Gisela
    Pinos, Tomas
    Marti, Ramon
    DiMauro, Salvatore
    Orhan Akman, Hasan
    Lucia, Alejandro
    Angel Martin, Miguel
    Arenas, Joaquin
    NEUROLOGY, 2009, 72 (11) : A17 - A17
  • [3] CONGENITAL ERYTHROPOIETIC PORPHYRIA: GENERATION AND CHARACTERIZATION OF KNOCK-IN MOUSE MODELS
    Bishop, D.
    Johansson, A.
    Desnick, R. J.
    JOURNAL OF INHERITED METABOLIC DISEASE, 2005, 28 : 258 - 258
  • [4] Characterization of a Novel Gain of Function Glucocorticoid Receptor Knock-in Mouse
    Zhang, Junhui
    Ge, Renshang
    Matte-Martone, Catherine
    Goodwin, Julie
    Shlomchik, Warren D.
    Mamula, Mark J.
    Kooshkabadi, Ali
    Hardy, Matthew P.
    Geller, David
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (10) : 6249 - 6259
  • [5] Generation and characterization of a Dkk4-Cre knock-in mouse line
    Khatif, Houda
    Bazzi, Hisham
    GENESIS, 2024, 62 (01)
  • [6] Generation and characterization of a Pax7-HA knock-in mouse line
    Hayashi, S.
    Inoue, Y.
    Kosako, H.
    Inoue, T.
    Noguchi, S.
    Nishino, I.
    NEUROMUSCULAR DISORDERS, 2021, 31 : S144 - S144
  • [7] Generation and characterization of PDGFR-GFPCreERt2 knock-In mouse line
    Miwa, Hiroyuki
    Era, Takumi
    GENESIS, 2015, 53 (05) : 329 - 336
  • [8] Generation and Characterization of Atoh1-Cre Knock-In Mouse Line
    Yang, Hua
    Xie, Xiaoling
    Deng, Min
    Chen, Xiaowei
    Gan, Lin
    GENESIS, 2010, 48 (06) : 407 - 413
  • [9] Generation and validation of a D1 dopamine receptor Flpo knock-in mouse
    Oppman, Alexis M.
    Paradee, William J.
    Narayanan, Nandakumar S.
    Kim, Young-cho
    JOURNAL OF NEUROSCIENCE METHODS, 2025, 415
  • [10] Androgen receptor knockout and knock-in mouse models
    Kerkhofs, S.
    Denayer, S.
    Haelens, A.
    Claessens, F.
    JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2009, 42 (1-2) : 11 - 17
12345