Targeted disruption of the mouse Lipoma Preferred Partner gene

被引：24
|
作者：
Vervenne, Hilke B. V. K. [1 ]
Crombez, Koen R. M. O. [1 ]
Delvaux, Els L. [1 ]
Janssens, Veerle [2 ]
de Ven, Wim J. M. Van [1 ]
Petit, Marleen M. R. [1 ]
机构：
[1] Katholieke Univ Leuven, Dept Human Genet, Mol Oncol Lab, Louvain, Belgium
[2] Katholieke Univ Leuven, Dept Mol Cell Biol, Prot Phosphorylat & Prote Lab, Louvain, Belgium
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 02期
关键词：
Zyxin; LPP; Lipoma Preferred Partner; TRIP6; ZRP-1; Knockout; Migration; Fertility; Female lethality; Aberrant pregnancy; SMOOTH-MUSCLE EXPRESSION; EXTENSION MOVEMENTS; FOCAL ADHESION; PROTEIN LPP; FUSION; ZYXIN; PEA3; MIGRATION; INTERACTS; CAPACITY;
D O I：
10.1016/j.bbrc.2008.12.074
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp(-/-) females. Fertility of Lpp(-/-) males was proven to be normal, however, females from Lpp(-/-) x Lpp(-/-) crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp(-/-) mice that were born reached adulthood without displaying any additional macroscopic defects. Oil the other hand, Lpp(-/-) mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：368 / 373
页数：6
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