Fabrication of a Modified Electrode Based on Fe3O4-Graphene Oxide Hybrid Composite: Applying to Simultaneous Determination of Adenine and Guanine in DNA

被引：10
|
作者：
Xie, Qianjie [2 ]
Chen, Xiang-yu [2 ]
Zhang, Hanzhi [2 ]
Liu, Mengdi [2 ]
Wang, Qian [3 ]
Zhang, Xin [1 ]
Shen, Yehua [2 ]
Yang, Fengchun [2 ]
机构：
[1] NW Univ Xian, Inst Analyt Sci, Shaanxi Prov Key Lab Electroanalyt Chem, Xian 710069, Shaanxi, Peoples R China
[2] NW Univ Xian, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Shaanxi, Peoples R China
[3] Xian Univ Posts & Telecommun, Sch Telecommun & Informat Engn, Xian 710121, Shaanxi, Peoples R China
来源：
ELECTROANALYSIS | 2015年 / 27卷 / 09期
关键词：
Fe3O4; nanoparticles; Graphene oxide; Adenine; Guanine; Simultaneous determination; DNA; GLASSY-CARBON ELECTRODE; ELECTROCHEMICAL SENSOR; MASS-SPECTROMETRY; ASCORBIC-ACID; URIC-ACID; GRAPHENE; NANOCOMPOSITE; NANOPARTICLES; FILM; OXIDATION;
D O I：
10.1002/elan.201500186
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A Fe3O4-graphene oxide hybrid composite (Fe3O4- GO) was synthesized and used to construct a novel electrochemical sensor for the simultaneous detection of adenine and guanine. The Fe3O4-GO based electrochemical sensor exhibits linear ranges of 0.05-25 mu M and 0.05-25 mu M with detection limits of 4 nM and 3 nM for adenine and guanine, respectively. This method was applied in the determination of guanine and adenine in many samples with satisfying results. The value of (G+C)/(A+T) in DNA was calculated to be 0.7536. The sensor exhibits some advantages, such as rapidity, good reproducibility and long-term stability.
引用
收藏
页码：2201 / 2208
页数：8
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