Denitrifying capability and community dynamics of glycogen accumulating organisms during sludge granulation in an anaerobic-aerobic sequencing batch reactor

被引:20
|
作者
Zhang Bin [1 ]
Xue Bin [1 ]
Qiu Zhigang [1 ]
Chen Zhiqiang [1 ]
Li Junwen [1 ]
Gong Taishi [1 ]
Zou Wenci [1 ]
Wang Jingfeng [1 ]
机构
[1] Inst Hlth & Environm Med, Tianjin Key Lab Risk Assessment & Control Environ, Tianjin 300050, Peoples R China
来源
SCIENTIFIC REPORTS | 2015年 / 5卷
基金
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词
BIOLOGICAL PHOSPHORUS REMOVAL; MICROBIAL-POPULATIONS; LABORATORY-SCALE; ACTIVATED-SLUDGE; SUBSTRATE UPTAKE; POLYPHOSPHATE; IDENTIFICATION; GRANULES; ALPHAPROTEOBACTERIA; ECOPHYSIOLOGY;
D O I
10.1038/srep12904
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Denitrifying capability of glycogen accumulating organisms (GAOs) has received great attention in environmental science and microbial ecology. Combining this ability with granule processes would be an interesting attempt. Here, a laboratory-scale sequencing batch reactor (SBR) was operated to enrich GAOs and enable sludge granulation. The results showed that the GAO granules were cultivated successfully and the granules had denitrifying capability. The batch experiments demonstrated that all NO3--N could be removed or reduced, some amount of NO2--N were accumulated in the reactor, and N-2 was the main gaseous product. SEM analysis suggested that the granules were tightly packed with a large amount of tetrad-forming organisms (TFOs); filamentous bacteria served as the supporting structures for the granules. The microbial community structure of GAO granules was differed substantially from the inoculant conventional activated sludge. Most of the bacteria in the seed sludge grouped with members of Proteobacterium. FISH analysis confirmed that GAOs were the predominant members in the granules and were distributed evenly throughout the granular space. In contrast, PAOs were severely inhibited. Overall, cultivation of the GAO granules and utilizing their denitrifying capability can provide us with a new approach of nitrogen removal and saving more energy.
引用
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页数:10
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