An improved deprotection procedure of amine-containing oligonucleotides from acrylonitrile modification

被引：4
|
作者：
Chang, CA [1 ]
Horn, T [1 ]
机构：
[1] Chiron Diagnostics, Emeryville, CA 94608 USA
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1999年 / 18卷 / 6-7期
关键词：
D O I：
10.1080/07328319908044663
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alkylation of alkylamine by acrylonitrile via Michael addition(1) occurred when standard ammonium hydroxide deprotection was used. The alkylation was greatly reduced using an improved two-step procedure when BCE phosphotriester protecting groups were selectively removed with t-butylamine prior to ammonium hydroxide deprotection.
引用
收藏
页码：1205 / 1206
页数：2
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