Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

被引:17
|
作者
Kubo, Yuji [1 ]
Inaoka, Takashi [2 ]
Hachiya, Tsuyoshi [3 ,4 ]
Miyake, Masayuki [3 ]
Hase, Sumitaka [3 ]
Nakagawa, Rikio [1 ]
Hasegawa, Hiromasa [1 ]
Funane, Kazumi [2 ]
Sakakibara, Yasubumi [3 ]
Kimura, Keitarou [2 ]
机构
[1] Ind Technol Inst Ibaraki Prefecture, Ibaraki 3113195, Japan
[2] NARO, NFRI, Div Appl Microbiol, Tsukuba, Ibaraki 3058642, Japan
[3] Keio Univ, Dept Biosci & Informat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan
[4] Iwate Med Univ, Iwate Med Megabank Org, Shiwa, Iwate 0283694, Japan
关键词
Bacillus subtilis; Rifampicin resistance; Colored soybean; Exoenzyme production; Natto; RNA-POLYMERASE MUTATION; PROTEIN; NEOTREHALOSADIAMINE; EXPRESSION; COMA; DEGU;
D O I
10.1016/j.jbiosc.2012.12.012
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the beta-subunit of the RNA polymerase. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
引用
下载
收藏
页码:654 / 657
页数:4
相关论文
共 50 条
  • [41] Evaluation of the utility of surfactin produced by the native strain of Bacillus subtilis natto BS19 in reducing the feeding and development of Oulema melanopus and Oulema gallaeciana
    Koim-Puchowska, Beata
    Lamparski, Robert
    Drozdz-Afelt, Joanna Maria
    JOURNAL OF PLANT PROTECTION RESEARCH, 2023, 63 (02) : 208 - 218
  • [42] Modeling and optimization of fermentation variables for enhanced production of lactase by isolated Bacillus subtilis strain VUVD001 using artificial neural networking and response surface methodology
    T. C. Venkateswarulu
    K. Vidya Prabhakar
    R. Bharath Kumar
    S. Krupanidhi
    3 Biotech, 2017, 7
  • [43] Modeling and optimization of fermentation variables for enhanced production of lactase by isolated Bacillus subtilis strain VUVD001 using artificial neural networking and response surface methodology
    Venkateswarulu, T. C.
    Prabhakar, K. Vidya
    Kumar, R. Bharath
    Krupanidhi, S.
    3 BIOTECH, 2017, 7
  • [44] Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an Engineered Bacillus subtilis Strain
    Sun, Xi
    Yang, Jun
    Fu, Xiaoping
    Zhao, Xingya
    Zhen, Jie
    Song, Hui
    Xu, Jianyong
    Zheng, Hongchen
    Bai, Wenqin
    BIOENGINEERING-BASEL, 2023, 10 (08):
  • [45] Peptide production through enhanced solid-state fermentation of soybean meal with Bacillus subtilis SBM_1: Fermentation process and product characteristics
    Liu D.
    Guo Y.
    Zhu J.
    Yolandani
    Wang Y.
    Ma H.
    Industrial Crops and Products, 2024, 219
  • [46] Enhanced Production of Poly-γ-glutamic Acid by Bacillus subtilis Using Stage-controlled Fermentation and Viscosity Reduction Strategy
    Yin Guo
    Yuanyuan Liu
    Zejian Yang
    Guiguang Chen
    Zhiqun Liang
    Wei Zeng
    Applied Biochemistry and Biotechnology, 2024, 196 : 1527 - 1543
  • [47] Enhanced Production of Poly-?-glutamic Acid by Bacillus subtilis Using Stage-controlled Fermentation and Viscosity Reduction Strategy
    Guo, Yin
    Liu, Yuanyuan
    Yang, Zejian
    Chen, Guiguang
    Liang, Zhiqun
    Zeng, Wei
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2024, 196 (03) : 1527 - 1543
  • [48] Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis
    Ozdemir, Sadin
    Guven, Kemal
    Baysal, Zuebeyde
    Uyar, Fikret
    FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2009, 47 (04) : 364 - 369
  • [49] ENHANCED-ALPHA - AMYLASE PRODUCTION BY CHROMOSOMAL INTEGRATION OF PTVA1 IN INDUSTRIAL STRAIN IN BACILLUS-SUBTILIS
    SLUGENOVA, M
    BUKOVSKA, G
    TURNA, J
    TIMKO, J
    BIOTECHNOLOGY LETTERS, 1993, 15 (05) : 483 - 488
  • [50] Production of amylase from Bacillus subtilis sp. strain KR1 under solid state fermentation on different agrowastes
    Pranay, Kumar
    Padmadeo, Shree Ram
    Prasad, Birendra
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2019, 21