Detection of DNA damage by alkaline comet assay in mouse colonic mucosa

被引：0
|
作者：
Boutet-Robinet, Elisa [1 ]
Haykal, Maria M. [2 ]
Hashim, Saleha [1 ]
Frisan, Teresa [3 ,4 ]
Martin, Oceane C. B. [5 ]
机构：
[1] Univ Toulouse, Res Ctr Food Toxicol, Toxalim, INRAE,ENVT,INP Purpan,UPS, Toulouse, France
[2] Univ Paris 12 Sud, Gustave Roussy Res Ctr, Dept Mol Med, INSERM U981,Labex LERMIT, Villejuif, France
[3] Umea Univ, Dept Mol Biol, Umea, Sweden
[4] Umea Univ, Umea Ctr Microbial Res UCMR, Umea, Sweden
[5] Univ Bordeaux, INSERM, BaRITOn, U1053, F-33000 Bordeaux, France
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 04期
基金：
瑞典研究理事会;
关键词：
Cell isolation; Cell-based Assays; Microscopy;
D O I：
10.1016/j.xpro.2021.100872
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We recently characterized the association between DNA damage and immunor-esponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimu-rium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]). For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).
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页数：13
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