Apoptosis of Hepatitis B Virus-expressing Liver Tumor Cells Induced by a High Concentration of Nucleos(t)ide Analogue

被引：3
|
作者：
Tak, Eunyoung [1 ]
Hwang, Shin [2 ]
Lee, Han Chu [3 ]
Ko, Gi-Young [4 ]
Ahn, Chul-Soo [2 ]
Yoon, Young-In [2 ]
Lim, Young-Suk [3 ]
Jun, Dae-Young [1 ]
Kim, Ki-Hun [2 ]
Song, Gi-Won [2 ]
Moon, Deog-Bok [2 ]
Ryoo, Baek-Yeol [5 ]
Kim, Nayoung [1 ]
Lee, Sung-Gyu [2 ]
机构：
[1] Univ Ulsan, Coll Med, Asan Med Ctr, Asan Inst Life Sci, Seoul, South Korea
[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Surg, 88 Olymp Ro 43 Gil, Seoul 138736, South Korea
[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Gastroenterol, Seoul, South Korea
[4] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Diagnost Radiol, Seoul, South Korea
[5] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Oncol, Seoul, South Korea
来源：
ANTICANCER RESEARCH | 2016年 / 36卷 / 11期
基金：
新加坡国家研究基金会;
关键词：
Tenofovir; entecavir; covalently closed circular DNA; HBV X protein; transcatheter arterial chemoembolization; TENOFOVIR DISOPROXIL FUMARATE; CLOSED CIRCULAR DNA; HEPATOCELLULAR-CARCINOMA; IMMUNOGLOBULIN MONOTHERAPY; HBSAG SEROCLEARANCE; CLINICAL-OUTCOMES; RECURRENCE; THERAPY; RISK; TRANSPLANTATION;
D O I：
10.21873/anticanres.11195
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: We investigated the expression of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and HBV X protein (HBx) in human hepatocellular carcinoma (HCC) and evaluated the effect of high-concentration nucleos(t)ide analogs (NUCs) on liver tumor cell lines. Materials and Methods: This study consisted of three parts: part I used human blood and non-tumor liver tissues; part II used human HCC and adjacent liver tissues; and part III used an HBV-expressing liver tumor cell line. Results: There were close correlations among blood and liver HBV DNA and liver cccDNA. HBV cccDNA and HBx were highly up-regulated in HCC compared to adjacent liver tissues despite NUC therapy. HBV cccDNA and HBx were highly up-regulated in the cccDNA-expressing HepG2.2.15 cell line. Their expression was down-regulated and apoptosis was induced by a very high concentration of NUCs in dose-and time-dependent manner. Conclusion: Very high concentrations of NUCs may have a novel potential to kill replicating HBV-expressing liver tumor cells.
引用
收藏
页码：6059 / 6069
页数：11
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