Factors influencing shoot multiplication of lotus (Nelumbo nucifera)

被引：18
|
作者：
Shou, S. -Y. [1 ]
Miao, L. -X. [1 ]
Zai, W. -S. [1 ,2 ]
Huang, X. -Z. [1 ]
Guo, D. -P. [1 ]
机构：
[1] Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China
[2] Wenzhou Acad Agr Sci, Wenzhou 325006, Zhejiang, Peoples R China
来源：
BIOLOGIA PLANTARUM | 2008年 / 52卷 / 03期
关键词：
auxins; cytokinins; in vitro culture; lotus; photoperiod; plant regeneration; temperature;
D O I：
10.1007/s10535-008-0103-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Effect of plant growth regulators, explant size, season of explant collection, temperature ( 20, 25 and 30 C) and photoperiod on in vitro lotus (Nelumbo nucifera Gaertn.) shoot formation and growth were examined. Shoots formation was greatly influenced by growth regulators, explant size and season of explant collection. The maximum number of shoots were induced from bud explants on Murashige and Skoog (MS) medium containing 4.44 mu M benzyladenine (BA) + 0.54 mu M alpha-naphthalene acetic acid (NAA). Explants formed by bud of one expanded and one unexpanded leaf, which was collected in spring gave encouraging results of shoot production. Higher temperature favoured shoot induction and subsequent growth was much better at 25 degrees C compared to that at 20 and 30 degrees C.
引用
收藏
页码：529 / 532
页数：4
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