Poly (C)-Binding Protein 1 Regulates p63 Expression through mRNA Stability

被引：42
|
作者：
Cho, Seong-Jun [1 ]
Jung, Yong-Sam [1 ]
Chen, Xinbin [1 ]
机构：
[1] Univ Calif Davis, Sch Vet Med, Comparat Oncol Lab, Davis, CA 95616 USA
来源：
PLOS ONE | 2013年 / 8卷 / 08期
基金：
美国国家卫生研究院;
关键词：
PROTECTS MOUSE OOCYTES; BINDING PROTEIN; P53; FAMILY; ENDORIBONUCLEASE ACTIVITY; TARGET GENES; RNPC1; APOPTOSIS; HUR; DIFFERENTIATION; INDUCTION;
D O I：
10.1371/journal.pone.0071724
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
p63, a transcription factor and p53 family protein, plays a crucial role in tumor suppression and development of various epithelial tissues. While p63 expression is controlled mostly by post-translational modifications, recent studies indicate that transcriptional and posttranscriptional regulations are essential for proper p63 expression. Here, we investigated the regulation of p63 expression by poly (C)-binding protein 1 (PCBP1, also known as hnRNP-E1 and alpha CP1). We found that knockdown of PCBP1 decreases the level of p63 transcript and protein. We also found that PCBP1 regulates the stability of p63 mRNA via binding to p63 3'UTR. Additionally, we found that a CU-rich element (CUE) in p63 3'UTR is bound by and responsive to PCBP1. Together, we conclude that PCBP1 regulates p63 expression via mRNA stability.
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页数：7
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