Simple method for transformation of Ochrobactrum anthropi

被引：3
|
作者：
Seleem, Mohamed N. [1 ,2 ]
Rajasekaran, Parthiban [1 ]
Ali, Mohammed [3 ]
Boyle, Stephen M. [1 ]
Sriranganathan, Nammalwar [1 ]
机构：
[1] Virginia Polytech Inst & State Univ, Dept Biomed Sci & Pathobiol, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA
[2] Virginia Polytech Inst & State Univ, Inst Crit Technol & Appl Sci, Blacksburg, VA 24061 USA
[3] Assiut Univ, Dept Forens Med & Toxicol, Fac Vet Med, Assiut, Egypt
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2008年 / 24卷 / 10期
关键词：
biodegradation; bioremediation; plasmid;
D O I：
10.1007/s11274-008-9716-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A rapid and simple method for preparation of highly efficient Ochrobactrum anthropi electrocompetent cells has been developed. The efficiency of transformation increased 200-fold when the cells were prepared from liquid culture compared to agar plates. Effects of different conditions, including cell density, electric field strength, resistance and plasmid size were evaluated to develop an electroporation protocol. The electrocompetent O. anthropi prepared by this method were 9-fold more efficient than commercial sources of competent Escherichia coli. The method described here will enhance the genetic manipulation of Ochrobactrum as a bioremediation tool and a biopesticide agent.
引用
收藏
页码：2111 / 2114
页数：4
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