Isolation and characterization of polymorphic microsatellite loci in the Hawaiian flycatcher, the elepaio (Chasiempis sandwichensis)

被引：3
|
作者：
Burgess, SL
Fleischer, RC
机构：
[1] Univ Hawaii, John A Burns Sch Med, Dept Cell & Mol Biol, Evolut Ecol & Conservat Biol Program, Honolulu, HI 96822 USA
[2] Smithsonian Inst, Museum Natl Hist Nat, Genet Program, Washington, DC 20008 USA
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 01期
关键词：
Chasiempis; elepaio; Hawaii; primers; microsatellites;
D O I：
10.1111/j.1471-8286.2005.01093.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thirteen polymorphic microsatellite loci were isolated and characterized from two clades of an endemic Hawaiian flycatcher, the elepaio (Chasiempis sandwichensis). Seven dinucleotide repeats and one trinucleotide repeat were cloned from Kauai elepaio; five dinucleotide repeats were cloned from Oahu elepaio. Polymorphism was assessed in a sample of Oahu elepaio (n = 22) revealing two to 16 alleles per locus. Observed heterozygosity ranged from 0.14 to 0.91. However, linkage analysis exposed highly significant linkage disequilibrium between two of the most polymorphic loci. Twelve loci are therefore expected to be useful for investigations of population structure.
引用
收藏
页码：14 / 16
页数：3
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