Sex-sorting sperm by flow cytometry in pigs: Issues and perspectives

被引：46
|
作者：
Vazquez, J. M. [1 ]
Parrilla, I. [1 ]
Roca, J. [1 ]
Gil, M. A. [1 ]
Cuello, C. [1 ]
Vazquez, J. L. [1 ]
Martinez, E. A. [1 ]
机构：
[1] Univ Murcia, Fac Vet Med, Dept Anim Med & Surg, E-30071 Murcia, Spain
来源：
THERIOGENOLOGY | 2009年 / 71卷 / 01期
关键词：
Sperm sexing; Swine; PSP-I/PSP-II; Deep intrauterine insemination; Laparoscopic insemination; SORTED BOAR SPERMATOZOA; DEEP INTRAUTERINE INSEMINATION; CHROMOSOME-BEARING SPERMATOZOA; LOW-DOSE INSEMINATION; IN-VITRO FERTILIZATION; PSP-I/PSP-II; SEMINAL PLASMA; ARTIFICIAL-INSEMINATION; FUNCTIONAL INTEGRITY; MAMMALIAN SPERM;
D O I：
10.1016/j.theriogenology.2008.09.044
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Several hundred thousand offspring of preselected sex of various species have been born since sperm sexing technology based on flow cytometric sorting of X- and Y-chromosome-bearing sperm and DNA was first demonstrated in 1989. The advantages derived from application of sexing technology to commercial dairy cattle production have been demonstrated worldwide. Utilizing sex-sorting technology for pig production systems offers many similar advantages. However, several factors currently limit implementation of sexing technology in pigs. Anatomical and physiological features inherent to the female pig, together with the relatively low sperm output of a flow sorter, are the main limitations to widespread use of this technology in pig production systems. This review analyzes the factors that limit the efficiency of sperm sorting technology for commercial swine production. In addition, this review discusses recent innovations in technical instrumentation and applied reproductive techniques that may help to overcome some of these limitations. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：80 / 88
页数：9
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