Adventitious Shoot Regeneration from Immature Embryo and Cotyledon Explants of Sunflower (Helianthus annuus L.)

被引:1
|
作者
Meral, Uelkue Binboga [1 ]
机构
[1] Gazi Univ, Fen Bilimleri Enstitusu, Ankara, Turkey
关键词
Helianthus annuus L; sunflower; immature embryo; immature cotyledons; in vitro;
D O I
10.1501/Tarimbil_0000000445
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Immature embryo and cotyledons of 10 lines of sunflower (Helianthus annuus L) were cultured on Murashige and Skoog (MS) medium supplemented with 20 mg/l sucrose and different concentrations of Kinetin and alpha Naphthalene acetic acid (NAA). Development of precocious roots was observed after one week of culture, which were removed aseptically to induce callus and shoot regeneration. Induction of healthy shoots was observed on cotyledon and embryo explants on MS medium containing any concentration of kinetin and and NAA. This is an important development in the tissue culture research of sunflower.
引用
收藏
页码:95 / 100
页数:6
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