Hyphal surface architecture and cell morphology of Trichoderma reesei

被引:4
|
作者
Nitta, Mikiko [1 ,2 ]
Shida, Yosuke [1 ]
Okada, Hitoshi [3 ]
Osumi, Masako [3 ,4 ]
Ogasawara, Wataru [1 ]
机构
[1] Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata 9402188, Japan
[2] Japan Sci & Technol Agcy JST, Kawaguchi, Saitama 3320012, Japan
[3] Non Profit Org Integrated Imaging Res Support IIR, Chiyoda Ku, Tokyo 1020093, Japan
[4] Japan Womens Univ, Lab Electron Microscopy, Bioimaging Ctr, Bunkyo Ku, Tokyo 1128681, Japan
来源
JOURNAL OF ELECTRON MICROSCOPY | 2012年 / 61卷 / 03期
关键词
cell wall; extracellular polysaccharide; fibrous layer; fibrous material; Trichoderma reesei; BETA-GLUCOSIDASE; ULTRASTRUCTURAL-LOCALIZATION; CELLULASE; SECRETION; MUTANT; WALLS;
D O I
10.1093/jmicro/dfs037
中图分类号
TH742 [显微镜];
学科分类号
摘要
The filamentous fungus Trichoderma reesei which is known to secrete high amounts of cellulolytic enzymes was found to produce a massive amount of fibrous material at the outer surface of the cell wall as observed by ultrahigh-resolution low-voltage scanning electron microscopy. Using transmission electron microscopy, the cell wall ornamentation of the hyper-cellulosic mutant PC-3-7 was found to be less massive and much thinner than for QM9414. A significant amount of fibrous material was produced in Avicel-grown cultures that were less abundant in glucose-grown cultures and Avicel was occasionally found entangled within the cell wall-associated fibrous layer.
引用
收藏
页码:187 / 192
页数:6
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