Effect of genotype and explant age on callus induction and subsequent plant regeneration from root-derived callus of Indica rice genotypes

被引：71
|
作者：
Hoque, ME
Mansfield, JW
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Agr Sci, Ashford TN25 5AH, Kent, England
[2] Bangladesh Rice Res Inst, Div Biotechnol, Gazipur 1701, Bangladesh
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 78卷 / 03期
关键词：
callus induction; genotypic variations; Indica rice; plant regeneration; root segments;
D O I：
10.1023/B:TICU.0000025640.75168.2d
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An attempt has been taken to establish an efficient plant regeneration system in vitro from 3, 5, 7 and 9-days-old root segments of four Indica (Bangladeshi) rice genotypes. Genotypic effects were observed in callus induction and subsequent plant regeneration. Moreover, the stage of development of the root explants also played a significant role in callus formation and subsequent plant regeneration. Younger explants were more efficient in both callus induction and plant regeneration. Plants regenerated in vitro were successfully established in soil and produced fertile seeds.
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页码：217 / 223
页数：7
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