Purification and characterization of ACC oxidase from Artocarpus altilis

被引：14
|
作者：
Williams, OJ
Golden, KD
机构：
[1] McGill Univ, Ste Anne De Bellevue, PQ H9X 3V9, Canada
[2] Univ W Indies, Biochem Sect, Dept Basic Med Sci, Kingston 7, Jamaica
来源：
PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2002年 / 40卷 / 04期
关键词：
1-aminocyclopropane-1-carboxylic acid; ACC oxidase; Artocarpus altilis; enzyme characterization; ethylene; plant hormone; ripening;
D O I：
10.1016/S0981-9428(02)01379-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase was purified to homogeneity from breadfruit (Artocarpus altilis) by a six-step chromatography procedure to yield an 87-fold increase in purification with a recovery of 9.5%. SDS-PAGE revealed that the purified enzyme had a molecular mass of 42.3 kDa and a Km of 28.2 muM. The enzyme showed marked inhibition by cobalt sulphate, sodium metabisulphite, sodium dithionite, n-propyl gallate, zinc sulphate and hydrogen peroxide. Dithiothreitol (DTT) enhanced enzyme activity when it was included in the assay medium. Optimum activity of ACC oxidase was obtained at a pH of 7.2 at 28 degreesC. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码：273 / 279
页数：7
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