Effects of potassium halides on bacterial bioluminescence

被引:15
|
作者
Gerasimova, MA
Kudryasheva, NS
机构
[1] Krasnoyarsk State Univ, Dept Quantum Elect, Krasnoyarsk 660041, Russia
[2] RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
基金
俄罗斯基础研究基金会;
关键词
bioluminescence; halides; heavy atom; inhibition; activation;
D O I
10.1016/S1011-1344(02)00240-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of potassium halides KCl, KBr and KI on NADH:FMN-oxidoreductase-luciferase bioluminescent coupled enzyme system were studied. The influence of salt additions on bioluminescence intensity and bioluminescence light yield was investigated. The inhibition and activation parameters of the salts were calculated using their dependencies on concentration of the salts. The correlation between the inhibition of bioluminescence intensity and the halide mass was demonstrated: the inhibiting ability of the salts increases with the increase of atomic weight of the anions. The inhibition parameters increase and the activation parameters decrease, accordingly. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:218 / 222
页数:5
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