Synthesis of pyridinone ribonucleoside 3'-O-phosphoramidites and their incorporation into oligoribonucleotides

被引：17
|
作者：
MatulicAdamic, J [1 ]
Gonzalez, C [1 ]
Usman, N [1 ]
Beigelman, L [1 ]
机构：
[1] RIBOZYME PHARMACEUT INC,DEPT CHEM & BIOCHEM,BOULDER,CO 80301
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 04期
关键词：
D O I：
10.1016/0960-894X(96)00030-3
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Protected pyridin-2- and pyridin-4-one ribonucleosides 3 and 9 were synthesized using a one-pot reaction of silylated bases with 1-O-acetyl-tri-O-benzoyl-beta-D-ribofuranose (2) in the presence of CF(3)SO(3)SiMe(3) The nucleosides were converted in 4 steps into 3'-O-phosphoramidites 7 and 11 which were incorporated into hammerhead ribozyme substrates using solid-phase phosphoramidite chemistry.
引用
收藏
页码：373 / 378
页数：6
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