Multiple thiol-anchor capped DNA-gold nanoparticle conjugates

被引：314
|
作者：
Li, Z
Jin, RC
Mirkin, CA
Letsinger, RL
机构：
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[2] Northwestern Univ, Inst Nanotechnol, Evanston, IL 60208 USA
来源：
NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 07期
关键词：
D O I：
10.1093/nar/30.7.1558
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.
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页码：1558 / 1562
页数：5
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