Resettable and enzyme-free molecular logic devices for the intelligent amplification detection of multiple miRNAs via catalyzed hairpin assembly

被引:21
|
作者
Zhang, Siqi [1 ]
Li, Kai-Bin [1 ]
Shi, Wei [1 ]
Zhang, Jie [1 ]
Han, De-Man [1 ]
Xu, Jing-Juan [2 ,3 ]
机构
[1] Taizhou Univ, Dept Chem, Jiaojiang 318000, Peoples R China
[2] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
[3] Nanjing Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
MICRORNA DETECTION; NUCLEIC-ACIDS; MAGNETIC BEADS; PROBE; COMPLEX; GATES; UNITS;
D O I
10.1039/c8nr10103e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The integration of multi-level DNA logic gates for biological diagnosis is far from being fully realized. In particular, the simplification of logical analysis to implement advanced logic diagnoses is still a critical challenge for DNA computing and bioelectronics. Here, we developed a magnetic bead/DNA system to construct a library of logic gates, enabling the sensing of multiplex target miRNAs. In this assay, the miRNA-catalyzed hairpin assembly (CHA) was successfully applied to construct two/three-input concatenated logic circuits with excellent specificity extended to design a highly sensitive multiplex detection system. Significantly, the CHA-based multiplex detection system can distinguish individual target miRNAs (such as miR-21, miR-155, and miR let-7a) under a logic function control, which presents great applications in the development of rapid and intelligent detection. Another novel feature is that the multiplex detection system can be reset by heating the output system and the magnetic separation of the computing modules. Overall, the proposed logic diagnostics with high amplification efficiency is simple, fast, low-cost, and resettable, and holds great promise in the development of biocomputing, multiparameter sensing, and intelligent disease diagnostics.
引用
收藏
页码:5048 / 5057
页数:10
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