Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems

被引：50

作者：

Nalinanon, Sitthipong ^{[1
]}

Benjakul, Soottawat ^{[1
]}

Visessanguan, Wonnop ^{[2
]}

Kishimura, Hideki ^{[3
]}

机构：

[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Klongluang 12120, Pathumthani, Thailand

[3] Hokkaido Univ, Res Fac Fisheries Sci, Lab Marine Prod & Food Sci, Hakodate, Hokkaido 0418611, Japan

来源：

PROCESS BIOCHEMISTRY | 2009年 / 44卷 / 04期

关键词：

Stomach; Tuna; Albacore; Aqueous two-phase system; Protease; Partitioning; POLY(ETHYLENE GLYCOL); POLYETHYLENE-GLYCOL; COLLAGEN EXTRACTION; ESCHERICHIA-COLI; ACID PROTEASE; PURIFICATION; PROTEINS; PEPSIN; PH; ULTRAFILTRATION;

D O I：

10.1016/j.procbio.2008.12.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partitioning of protease from stomach of albacore tuna using an aqueous two-phase system (ATPS) was investigated. The best ATPS conditions for protease partitioning from stomach extract (SE) and acidified counterpart (ASE) were 25% PEG1000-20% MgSO4 and 15% PEG2000-15% MgSO4, Which increased the purity by 7.2-fold and 2.4-fold with the recovered activity of 85.7% and 89.1%, respectively. Electrophoretic study revealed that SE had a major protein with a molecular weight (MW) of 40.6 kDa, while protein with MW of 32.7 kDa was predominant in ASE and ATPS fractions. Pepsinogen in SE might be activated to pepsin by acidification and partitioning process. SE was quite stable at 0 and 4 degrees C Up to 14 days. The loss in protease activity in ASE and selected ATPS fractions was more pronounced when storage time and temperature increased. Therefore, ATPS can be effectively used to recover and purify protease from albacore tuna stomach. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：471 / 476

页数：6

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