Human IgM monoclonal antibody to ganglioside GM2 and complement suppress virus propagation in ex vivo cultures of lymphocytes from HIV-1 infected patients

被引：7
|
作者：
Okada, N
Wu, XS
Mizokami, M
Irie, RF
Okada, H
机构：
[1] Nagoya City Univ, Sch Med, Dept Biol Mol, Nagoya, Aichi 4678601, Japan
[2] Nagoya City Univ, Sch Med, Dept Internal Med 2, Nagoya, Aichi 4678601, Japan
[3] John Wayne Canc Inst, Dept Biotechnol Sci, Santa Monica, CA 90404 USA
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1999年 / 43卷 / 07期
关键词：
HIV; IgM; ex vivo; complement;
D O I：
10.1111/j.1348-0421.1999.tb02462.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
HIV-1 infection induces aberrant ganglioside GM2 expression on infected cell lines, and human IgM anti-GM2 monoclonal antibody (L55 Ab) together with normal fresh human serum (FHS) as a source of complement causes complement mediated cytolysis of HIV-1 infected cells as well as HIV-1 particles. We report here that high expression of GM2 was also detected on HIV-1 infected lymphocytes from HIV-1 seropositive patients. L55 Ab effectively suppressed the generation of HIV in the presence of FHS in primarily cultured lymphocytes from HIV-1 infected patients in ex vivo experiments, and the suppression was enhanced additively by AZT. These data suggest that L55 Ab may increase the therapeutic effect of chemotherapy.
引用
收藏
页码：723 / 727
页数：5
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