Advanced 3D Spheroid Culture for Evaluation of Photodynamic Therapy in Microfluidic System

被引：4
|
作者：
Zuchowska, A. [1 ]
Jastrzebska, E. [1 ]
Chudy, M. [1 ]
Dybko, A. [1 ]
Brzozka, Z. [1 ]
机构：
[1] Warsaw Univ Technol, Inst Biotechnol, Dept Microbioanalyt, Warsaw, Poland
来源：
PROCEEDINGS OF THE 30TH ANNIVERSARY EUROSENSORS CONFERENCE - EUROSENSORS 2016 | 2016年 / 168卷
关键词：
Lab-on-a-chip; spheroid; photodynamic therapy; long-term culture; CELLS;
D O I：
10.1016/j.proeng.2016.11.184
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In this paper we present A549 (carcinoma) and MRC-5 (non-malignant) long-term (10 days) spheroids culture in a microfluidic system. Moreover, the evaluation of photodynamic therapy (PDT) procedure with 5-aminolevulinic acid (ALA) on 3D A549 and MRC-5 spheroids culture was performed. It was found that the spheroids are more resistant for ALA/PDT than monolayer culture. Photocytotoxic effect of the tested ALA concentration on A549 spheroids was noticed 8 days after PDT procedure. Selective effect of PDT procedure on carcinoma cells was observed. (C) 2016 The Authors. Published by Elsevier Ltd.
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页码：403 / 406
页数：4
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