Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine

被引：59
|
作者：
Henao-Escobar, W. [1 ]
del Torno-de Roman, L. [1 ]
Dominguez-Renedo, O. [1 ]
Alonso-Lomillo, M. A. [1 ]
Arcos-Martinez, M. J. [1 ]
机构：
[1] Univ Burgos, Dept Analyt Chem, Fac Sci, Burgos 09001, Spain
来源：
FOOD CHEMISTRY | 2016年 / 190卷
关键词：
Dual enzymatic biosensor; Histamine; Putrescine; Screen-printed electrodes; Simultaneous determination; PRINTED CARBON ELECTRODES; BIOGENIC-AMINES; ACID DETERMINATION; DIAMINE OXIDASE; WINES; FOOD;
D O I：
10.1016/j.foodchem.2015.06.035
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A disposable electrodic system consisting of two working electrodes connected in array mode has been developed for the simultaneous determination of histamine (His) and putrescine (Put). Histamine deshydrogenase and putrescine oxidase enzymes were respectively immobilized by crosslinking on each working screen-printed electrode, both modified with tetrathiafulvalene. The dual system allowed the simultaneous amperometric determination of both species by measuring the oxidation current of the mediator in each working electrode. The effect of other potentially interfering biogenic amines was also evaluated. The capability of detection was of 8.1 +/- 0.7 for His and 10 +/- 0.6 mu M for Put. The precision in terms of relative standard deviation was of 3.5% and 6.7% for His and Put, respectively. The developed biosensor was successfully applied to the determination of His and Put in different food samples. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：818 / 823
页数：6
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