High-speed, miniaturized fluorescence microscopy in freely moving mice

被引：274
|
作者：
Flusberg, Benjamin A. [1 ]
Nimmerjahn, Axel [1 ]
Cocker, Eric D. [1 ]
Mukamel, Eran A. [1 ]
Barretto, Robert P. J. [1 ]
Ko, Tony H. [1 ]
Burns, Laurie D. [1 ]
Jung, Juergen C. [1 ]
Schnitzer, Mark J. [1 ,2 ]
机构：
[1] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA
[2] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
来源：
NATURE METHODS | 2008年 / 5卷 / 11期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
D O I：
10.1038/nmeth.1256
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca2+ dynamics.
引用
收藏
页码：935 / 938
页数：4
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