High-speed volumetric imaging of neuronal activity in freely moving rodents

被引：141

作者：

Skocek, Oliver ^{[1
]}

Nobauer, Tobias ^{[1
]}

Weilguny, Lukas ^{[2
]}

Traub, Francisca Martinez ^{[1
]}

Xia, Chuying Naomi ^{[1
]}

Molodtsov, Maxim, I ^{[2
]}

Grama, Abhinav ^{[3
,4
]}

Yamagata, Masahito ^{[3
,4
]}

Aharoni, Daniel ^{[5
]}

Cox, David D. ^{[3
,4
]}

Golshani, Peyman ^{[5
,6
,7
]}

Vaziri, Alipasha ^{[1
,2
,8
]}

机构：

[1] Rockefeller Univ, Lab Neurotechnol & Biophys, 1230 York Ave, New York, NY 10021 USA

[2] Res Inst Mol Pathol, Vienna, Austria

[3] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[4] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA

[5] Univ Calif Los Angeles, Brain Res Inst, Integrat Ctr Learning & Memory, Dept Neurol, Los Angeles, CA 90024 USA

[6] Univ Calif Los Angeles, Brain Res Inst, Integrat Ctr Learning & Memory, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90024 USA

[7] West Los Angeles Virginia Med Ctr, Los Angeles, CA USA

[8] Rockefeller Univ, Kavli Neural Syst Inst, 1230 York Ave, New York, NY 10021 USA

来源：

NATURE METHODS | 2018年 / 15卷 / 06期

基金：

美国国家科学基金会;

关键词：

LIGHT-FIELD MICROSCOPY; NEURAL ACTIVITY; BRAIN; RESOLUTION; DECONVOLUTION; MICE;

D O I：

10.1038/s41592-018-0008-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 x 600 x 360 mu m(3) at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as similar to 15 mu m and at depths up to 360 mu m can be discriminated.

引用

页码：429 / +

页数：6

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