Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

被引：20

作者：

de Medeiros, Gustavo ^{[1
,6
]}

Kromm, Dimitri ^{[1
,3
]}

Balazs, Balint ^{[1
,4
]}

Norlin, Nils ^{[1
]}

Guenther, Stefan ^{[1
]}

Izquierdo, Emiliano ^{[1
]}

Ronchi, Paolo ^{[1
]}

Komoto, Shinya ^{[1
,7
]}

Krzic, Uros ^{[1
]}

Schwab, Yannick ^{[1
]}

Peri, Francesca ^{[1
,5
]}

de Renzis, Stefano ^{[1
]}

Leptin, Maria ^{[1
]}

Rauzi, Matteo ^{[1
,2
]}

Hufnagel, Lars ^{[1
]}

机构：

[1] European Mol Biol Lab Heidelberg, Meyerhofstr 1, D-69117 Heidelberg, Germany

[2] Univ Cote Azur, CNRS, INSERM, iBV, Nice, France

[3] Collaborat Joint PhD Degree EMBL & Heidelberg Uni, Fac Biosci, Heidelberg, Germany

[4] Luxendo GmbH, Kurfursten Anlage 58, D-69115 Heidelberg, Germany

[5] Univ Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland

[6] Friedrich Miescher Inst Biomed Res, Maulbeerstr 66, CH-4058 Basel, Switzerland

[7] Okinawa Inst Sci & Technol, Imaging Sect, 1919-1 Tancha, Okinawa 9040495, Japan

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

CORTICAL FORCES; ABLATION; EMBRYOS; MICROSURGERY; CYTOSKELETON; NEURONS; SHAPE; DEEP;

D O I：

10.1038/s41598-019-54349-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

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页数：12

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