Expression of lactate dehydrogenase C in MDA-MB-231 cells and its role in tumor invasion and migration

被引：30
|
作者：
Kong, Lingying [1 ]
Du, Wei [1 ]
Cui, Zhaolei [2 ]
Wang, Li [1 ]
Yang, Zhi [1 ]
Zhang, Hongsheng [1 ]
Lin, Donghong [2 ]
机构：
[1] Fujian Univ Tradit Chinese Med, Affiliated Peoples Hosp, Dept Pathol, 602 817 Zhong Rd, Fuzhou 350004, Fujian, Peoples R China
[2] Fujian Med Univ, Dept Clin Lab, Sch Med Technol & Engn, Fuzhou 350004, Fujian, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2016年 / 13卷 / 04期
关键词：
lactate dehydrogenase C; breast cancer; MDA-MB-231; invasion; migration; CANCER-CELLS; LDH-A; GENE; SPERM; METABOLISM; INHIBITORS; APOPTOSIS; ANALOGS; TARGET; GROWTH;
D O I：
10.3892/mmr.2016.4963
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The cancer/testis antigen (CTA) lactate dehydrogenase C (LDHC) is a unique LDH isoenzyme associated with glucose and adenosine triphosphate production in mammalian germ cells. However, the role of LDHC in cancer has thus far largely remained elusive. The present study described the expression status of LDHC in human MDA-MB-231 breast cancer cells as well as its role in tumor invasion and migration. Immunohistochemical analysis revealed endogenous LDHC expression in the cytoplasm and nuclei of MDA-MB-231 cells yielded. In addition, in vitro cell invasion and migration assays revealed that when LDHC expression was blocked by its specific inhibitor, cell invasion and migration were compromised in MDA-MB-231 cells. Of note, inhibition of LDHC was unable to induce apoptosis in MDA-MB-231 cells. The present study provided evidence that the LDHC enzyme acts as a CTA in breast carcinoma and exerts an essential role in tumor invasion and migration.
引用
收藏
页码：3533 / 3538
页数：6
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