IMPROVEMENT OF LIPASE PRODUCTION FROM Geotrichum sp IN SHAKEN FLASKS

被引：8

作者：

Maldonado, Rafael Resende ^{[1
]}

Panciera, Andre Luiz ^{[1
]}

Macedo, Gabriela Alves ^{[2
]}

Mazutti, Marcio A. ^{[3
]}

Maugeri, Francisco ^{[1
]}

Rodrigues, Maria Isabel ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Food Engn, BR-13083862 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Dept Food Sci, BR-13083862 Campinas, SP, Brazil

[3] Univ Fed Santa Maria, Dept Chem Engn, BR-97105900 Santa Maria, RS, Brazil

来源：

CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY | 2012年 / 18卷 / 03期

关键词：

lipase; corn steep liquor Geotrichum sp; soybean oil; factorial design; CELL-BOUND LIPASE; OPTIMIZATION; PURIFICATION; CANDIDUM;

D O I：

10.2298/CICEQ120124022M

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This work is focused on the study of different variables on inoculum build-up aiming to improve the lipase production by Geotrichum sp. by means a sequential strategy of experimental design. The effects of inoculum size, corn steep liquor concentration, volume of inoculum, pH of medium, age of inoculum and soybean oil concentration on lipase activity were assessed by means of two factorial experimental designs. A maximum lipase activity of 35.20 +/- 0.8 U/mL was obtained with a inoculum composed of one circular area of 0.78 cm(2) containing spores, 50 mL of inoculum volume medium, 12 hours of inoculum age, 15% w/v of corn steep liquor concentration, 1.0% w/v of soybean oil concentration and initial pH 5.0 at 30 degrees C and 150 rpm in flasks. This work showed that an enhancement of lipase activity can be obtained using a sequential statistical factorial approach to define the variables for inoculum build-up.

引用

页码：459 / 464

页数：6

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