Evaluation of lipase production by Geotrichum candidum in shaken flasks and bench-scale stirred bioreactor using different impellers

被引：15

作者：

Maldonado, Rafael Resende ^{[1
]}

Medeiros Burkert, Janaina Fernandes ^{[2
]}

Mazutti, Marcio A. ^{[3
]}

Maugeri, Franciso ^{[1
]}

Rodrigues, Maria Isabel ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Food Engn, BR-13083862 Campinas, SP, Brazil

[2] Fed Univ Rio Grande, Coll Chem & Food, Rio Grande, RS, Brazil

[3] Univ Fed Santa Maria, Dept Chem Engn, Av Roraima,1000, BR-97105900 Santa Maria, RS, Brazil

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2012年 / 1卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Lipase; Geotrichum candidum; Experimental design; Medium optimization;

D O I：

10.1016/j.bcab.2012.01.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This work is focused on the evaluation of lipase production by Geotrichum candidum in shaken-flasks and bench-scale bioreactor using different impellers. The effects of temperature, initial pH, peptone, NaNO3, MgSO4 and soy oil concentrations in the enzyme production were evaluated using the experimental design methodology. The optimum conditions in shaken flasks were 3.0% of peptone and 0.5% of soy oil, with initial pH of 7.0, 30 degrees C and 250 rpm reaching approximately 16 U mL(-1). The lipase production was also studied in a bench-scale stirred bioreactor, where the Rushton turbine and pitched blade up were appropriated impellers for this process. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：147 / 151

页数：5

共 15 条

[1] Application of Yeast Hydrolysate in Extracellular Lipase Production by Geotrichum candidum in Shaken Flasks, Stirred Tank, and Airlift Reactors
Maldonado, Rafael Resende
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