Development of a three-dimensional microfluidic system for long-term tumor spheroid culture

被引：25
|
作者：
Ziolkowska, Karina [1 ]
Kwapiszewski, Radostaw [1 ]
Stelmachowska, Agnieszka [1 ]
Chudy, Michat [1 ]
Dybko, Artur [1 ]
Brzozka, Zbigniew [1 ]
机构：
[1] Warsaw Univ Technol, Dept Microbioanalyt, Fac Chem, PL-00664 Warsaw, Poland
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 173卷
关键词：
Lab-on-a-chip; Tumor spheroid; Long-term cell culture; Three-dimensional microfluidic system; CANCER; CELLS; 3D;
D O I：
10.1016/j.snb.2012.07.045
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this paper, we present a low-cost three-dimensional microfluidic system made of poly(dimethylsiloxane) for tumor spheroid formation and cultivation. Tumor spheroids reaching diameters of up to 300 mu m were cultured in perfusion microchambers providing extremely low shear stress. Spheroids were cultured for 15 days and high viability of the cells was observed within this time. The presented microsystem is a prospective solution for development of microfluidic platforms for anticancer drug screening. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：908 / 913
页数：6
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