Characterization of Monoclonal Antibodies that Recognize the Eimeria tenella Microneme Protein MIC2

The apicomplexan pathogens of Eimeria tenella cause coccidiosis, ill intestinal disease of chickens that has a major economic impact on the poultry industry. Members of Apicomplexa share an assortment of unique secretory organelles (rhoptries, micronemes, and dense granules) that mediate invasion of host cells and the formation and modification of the parasitophorous vacuole. Among these, microneme protein 2 front E. tenella (EtMIC2) has a putative function in parasite adhesion to the host cell to initiate file invasion process. To investigate the role of EtMIC2 in host parasite interactions, the production and characterization of 12 monoclonal antibodies (mabs) produced against recombinant EtMIC2 proteins is described. In an immunofluoresence assay, all mabs reacted with molecules belonging to the apical complex of sporozoites and merozoites of L tenella, E. acervulina, and E. maxima. By Western blot analysis, the mabs identified it developmentally regulated protein of 42 kDa corresponding, to EtMIC2 and cross-reacted with Proteins in developmental stages of F. acervulina. Collectively these Mails are useful tools for the detailed investigation of, file Characterization of EtMIC-2-related proteins ill Eimeria species.