Optimization of micronutrients for the improvement of in vitro plant regeneration of Stevia rebaudiana (Bert.) Bertoni

被引:0
|
作者
Jain, Pourvi [1 ]
Kachhwaha, Sumita [1 ]
Kothari, S. L. [1 ]
机构
[1] Univ Rajasthan, Dept Bot, Jaipur 302004, Rajasthan, India
来源
INDIAN JOURNAL OF BIOTECHNOLOGY | 2012年 / 11卷 / 04期
关键词
Chlorophyll; clonal plants; micronutrients; micropropagation; Stevia rebaudiana; COPPER; L;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Optimization of micronutrients In the MS basal culture medium resulted into enhanced in vitro plant regeneration, chlorophyll content and biomass in Stevia rebaudiana (Bert.) Bertoni. The shoot bud induction response improved on medium with increased levels of MnSO4 (400 mu M), KI (10 mu M) and CoCl2 (2 mu M). Plant regenerated on higher levels of micronutrients showed significant increase in leaf chlorophyll content. The cumulative effect of all micronutrients at the optimum level in the composite medium also positively influenced the biomass and chlorophyll content of the plantlets.
引用
收藏
页码:486 / 490
页数:5
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