Cholinesterase biosensor construction - A review

被引：29
|
作者：
Pohanka, Miroslav [2 ]
Jun, Daniel [1 ,3 ]
Kalasz, Huba [4 ,5 ]
Kuca, Kamil [1 ,3 ,6 ]
机构：
[1] Univ Def, Ctr Adv Studies, Fac Mil Med, Hradec Kralove, Czech Republic
[2] Cent Mil Inst Hlth, Ctr Biol Def, Techonin, Czech Republic
[3] Univ Def, Dept Toxicol, Fac Mil Med, Hradec Kralove, Czech Republic
[4] Semmelweis Univ, Dept Pharmacodynam, H-1085 Budapest, Hungary
[5] UAE Univ, Dept Pharmacol & Therapeut, FMHS, Al Ain, U Arab Emirates
[6] Univ JE Purkyne, Dept Chem, Fac Sci, Usti Nad Labem, Czech Republic
来源：
PROTEIN AND PEPTIDE LETTERS | 2008年 / 15卷 / 08期
关键词：
cholinesterase; immobilization; biosensor; carbamate; organophosphate; pesticide;
D O I：
10.2174/092986608785203665
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Biosensors using cholinesterases as the biorecognition component have been used to assay organophosphates and carbamates for a long time. In this review, some strategies convenient for biosensor construction are presented. Solutions for cholinesterase immobilization and output signal monitoring are presented as the basic presumptions for successful biosensor construction.
引用
收藏
页码：795 / 798
页数：4
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