TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food

被引：29
|
作者：
Lambertz, S. Thisted [1 ]
Nilsson, C. [1 ]
Hallanvuo, S. [2 ]
机构：
[1] Natl Food Adm Toxicol Lab, Dept Res & Dev, SE-75126 Uppsala, Sweden
[2] Environm & Food Res Lab TavastLab, Hameenlinna, Finland
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 20期
关键词：
D O I：
10.1128/AEM.01459-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.
引用
收藏
页码：6465 / 6469
页数：5
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