Screening and validation for core genes in osteoarthritic cartilage based on weighted gene co-expression network analysis

被引:0
|
作者
Wang, S. -Q. [1 ]
Xie, W. -P. [2 ]
Yue, L. [2 ]
Cai, Y. -L. [1 ,2 ]
机构
[1] Shandong Univ Tradit Chinese Med, Clin Coll 1, Jinan, Shandong, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Dept Orthoped, Affiliated Hosp, Jinan, Shandong, Peoples R China
关键词
Osteoarthritis cartilage; Weighted gene co-expres-sion network analysis; Differentially expressed genes; Bioinformatics analysis; Immune infiltration; GADD45-BETA; TRANSCRIPTION; EXPRESSION; CHONDROCYTES; DEFICIENCY;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
- OBJECTIVE: Osteoarthritis (OA) has the highest disability rate among chron-ic diseases. The burden on patients and pub-lic health care resources is increasingly evident due to increasing obesity rates and aging popu-lations. So, there is still a lack of early diagnosis and treatment for OA. MATERIALS AND METHODS: A total of three OA cartilage tissue datasets (GSE1919, GSE32317, and GSE5235) were obtained from the Gene Ex-pression Omnibus (GEO) database. Screening of differentially expressed genes and WGCNA of overlapping genes were performed using the R language package. Functional and immune in-filtration analyses of overlapping genes were al- so carried out while hub genes were screened through LASSO regression analysis method and ROC curve. Finally, experimental validation was carried out through PCR and Western Blot analy-sis of rat cartilage. RESULTS: A total of 149 differentially ex-pressed genes were screened, and they were mainly enriched in the cytokine-cytokine recep-tor interaction, rheumatoid arthritis, and inter-leukin (IL-17) signaling pathways. Four co -ex-pression modules were obtained, of which the blue module was the most substantial morbidity associated with OA. Thirteen overlapping genes were identified based on significant module net- work topology analysis and differential genes, upon which their validation through LASSO re-gression analysis method and ROC curve was performed. From these, five signature genes were determined, before three potential core genes were finally identified after confirmation using the validation set. CONCLUSIONS: ATF3, FOSL2, and GADD45B may be hub genes to the osteochondropathy, and they are expected to be new biomarkers and drug targets in OA research.
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收藏
页码:8234 / 8246
页数:13
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