Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity

被引：35

作者：

Gaster, Richard S. ^{[2
,3
]}

Hall, Drew A.

Wang, Shan X. ^{[1
,4
]}

机构：

[1] Stanford Univ, Geballe Lab Adv Mat, Dept Elect Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[3] Stanford Univ, Med Scientist Training Program, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA

来源：

NANO LETTERS | 2011年 / 11卷 / 07期

基金：

美国国家科学基金会;

关键词：

Biosensors; nanobiotechnology; antibody cross-reactivity; giant magnetoresistance; magnetic nanoparticles; SPIN-VALVE SENSORS; MICROARRAYS; PROTEOMICS; ANTIGEN;

D O I：

10.1021/nl1026056

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.

引用

页码：2579 / 2583

页数：5

共 50 条

[31] A tumor-binding antibody with cross-reactivity to viral antigens
Campa, Michael J.
Gottlin, Elizabeth B.
Wiehe, Kevin
Patz Jr, Edward F.
CANCER IMMUNOLOGY IMMUNOTHERAPY, 2025, 74 (04)
[32] Development of a displacement immunoassay by exploiting cross-reactivity of a monoclonal antibody
Gerdes, M
Meusel, M
Spencer, F
ANALYTICAL BIOCHEMISTRY, 1997, 252 (01) : 198 - 204
[33] ANTIBODY SPECIFICITY - A RESULT OF CROSS-REACTIVITY OF ANTIGEN WITH IMMUNOCOMPETENT CELLS
SIEGEL, I
JOURNAL OF CELL BIOLOGY, 1968, 39 (2P2): : A124 - &
[34] Antibody cross-reactivity and the viral aetiology of type 1 diabetes
Coppieters, Ken T.
von Herrath, Matthias
JOURNAL OF PATHOLOGY, 2013, 230 (01): : 1 - 3
[35] Antibody cross-reactivity in pig organ xenotransplantation: how important is it?
Habibabady, Zahra
Kinoshita, Kohei
Hara, Hidetaka
Pierson, Richard N., III
Cooper, David K. C.
TRANSPLANTATION, 2023, 107 (10) : 25 - 25
[36] Methods and protocols for the assessment of protein allergenicity and cross-reactivity
Tong, Joo Chuan
Tammi, Martti Tapani
FRONTIERS IN BIOSCIENCE-LANDMARK, 2008, 13 : 4882 - 4888
[37] Corticosteroid cross-reactivity
Isaksson, M
Bruze, M
CONTACT DERMATITIS, 2003, 49 (01) : 53 - 54
[38] Cross-reactivity in Horses
Dutau, Guy
REVUE FRANCAISE D ALLERGOLOGIE, 2013, 53
[39] CROSS-REACTIVITY OF ANTIBODY TO POKEWEED ANTIVIRAL PROTEIN-I (PAPI) WITH PAPII, PAPS, AND RICIN-A
PRESTON, JF
ERVIN, SE
FEDERATION PROCEEDINGS, 1987, 46 (06) : 2083 - 2083
[40] A Case of Cross-Reactivity
Korswagen, Hendrik C.
CHEMISTRY & BIOLOGY, 2011, 18 (04): : 409 - 410

← 1 2 3 4 5 →