Experimental infection of foot and mouth disease virus (FMDV) upregulates the expression of Coxsackie and adenovirus receptor (CAR) in the myocardium of suckling mice

被引：0
|
作者：
Priyanka, Mahadappa [1 ]
Ranjitha, H. B. [1 ]
Karikalan, M. [2 ]
Chandramohan, S. [1 ]
Behera, Subhasmitha [1 ]
Gnanavel, V. [1 ]
Periyasamy, Tamil Selvan Ramasamy [1 ]
Umapathi, V. [1 ]
Dechamma, H. J. [1 ]
Krishnaswamy, Narayanan [1 ]
机构：
[1] ICAR Indian Vet Res Inst, Reg Campus, Bengaluru 24, Karnataka, India
[2] Deemed Univ, ICAR Indian Vet Res Inst, Ctr Wildlife Conservat Management & Dis Surveillan, Bareilly 243122, India
来源：
MICROBIAL PATHOGENESIS | 2023年 / 184卷
关键词：
Coxsackie and adenoviral receptor; Foot and mouth disease virus; Myocarditis; DILATED CARDIOMYOPATHY; B VIRUSES; PCR; PATHOGENESIS; STRAINS; DEATH;
D O I：
10.1016/j.micpath.2023.106383
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The relative overexpression of Coxsackie and adenoviral receptor (CAR) predisposes children to viral myocarditis. As the foot and mouth disease virus (FMDV) causes fatal myocarditis in calves, lambs, and piglets and belongs to the same family as the Coxsackie virus, we investigated the role of CAR in FMDV induced myocarditis in the suckling mice model. Swiss albino suckling mice of 5 days (n = 24) were divided into two equal groups. One group was inoculated with suckling mice adapted FMDV serotype O at 10 LD50, while the other group served as uninfected control. In addition, adult mice (n = 12) served as the control for age related CAR expression and lack of pathogenicity to FMDV. The establishment of myocarditis was confirmed by histopathological changes typical of myocarditis along with immunolocalization of FMDV antigens in the heart of suckling mice. The FMDV inoculated suckling mice group showed a significant upregulation of CAR transcripts by 2.5 folds, overexpression of CAR protein by densitometric analysis of immunoblots, and intense immunolocalization of CAR in the sarcolemma and intercalated discs of cardiomyocytes as compared to the uninfected suckling mice group and adult mice. It was concluded that FMDV infection induced overexpression of CAR in the myocardium of suckling mice.
引用
收藏
页数：7
相关论文
共 50 条
  • [41] Protection of mice against challenge with foot and mouth disease virus (FMDV) by immunization with foliar extracts from plants infected with recombinant tobacco mosaic virus expressing the FMDV structural protein VP1
    Wigdorovitz, A
    Filgueira, DMP
    Robertson, N
    Carrillo, C
    Sadir, AM
    Morris, TJ
    Borca, MV
    VIROLOGY, 1999, 264 (01) : 85 - 91
  • [42] Evidence for positive selection in the capsid protein-coding region of the foot-and-mouth disease virus (FMDV) subjected to experimental passage regimens
    Fares, MA
    Moya, A
    Escarmís, C
    Baranowski, E
    Domingo, E
    Barrio, E
    MOLECULAR BIOLOGY AND EVOLUTION, 2001, 18 (01) : 10 - 21
  • [43] DETERMINATION OF THE OPTIMAL AGE RANGE OF MICE FOR USE IN EXPERIMENTAL STUDIES WITH FOOT-AND-MOUTH DISEASE VIRUS
    GRAVES, JH
    POPPENSIEK, GC
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1960, 21 (83) : 694 - 696
  • [44] An adenovirus vectored mucosal adjuvant augments protection of mice immunized intranasally with an adenovirus-vectored foot-and-mouth disease virus subunit vaccine
    Alejo, Diana M.
    Moraes, Mauro P.
    Liao, Xiaofen
    Dias, Camila C.
    Tulman, Edan R.
    Diaz-San Segundo, Fayna
    Rood, Debra
    Grubman, Marvin J.
    Silbarte, Lawrence K.
    VACCINE, 2013, 31 (18) : 2302 - 2309
  • [45] Recovery of infectious foot-and-mouth disease virus from suckling mice after direct inoculation with in vitro-transcribed RNA
    Baranowski, E
    Molina, N
    Núñez, JI
    Sobrino, F
    Sáiz, M
    JOURNAL OF VIROLOGY, 2003, 77 (20) : 11290 - 11295
  • [46] An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV
    Guzman, Efrain
    Taylor, Geraldine
    Charleston, Bryan
    Skinner, Michael A.
    Ellis, Shirley A.
    JOURNAL OF GENERAL VIROLOGY, 2008, 89 : 667 - 675
  • [47] Recombinant adenovirus co-expressing capsid proteins of two serotypes of foot-and-mouth disease virus (FMDV): in vitro characterization and induction of neutralizing antibodies against
    Wu, QH
    Moraes, MP
    Grubman, MJ
    VIRUS RESEARCH, 2003, 93 (02) : 211 - 219
  • [48] Cell mediated innate responses of cattle and swine are diverse during foot-and-mouth disease virus (FMDV) infection: A unique landscape of innate immunity
    Toka, Felix N.
    Golde, William T.
    IMMUNOLOGY LETTERS, 2013, 152 (02) : 135 - 143
  • [49] Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice
    Guo, Zijing
    Zhao, Yin
    Zhang, Zhidong
    Li, Yanmin
    VIRUSES-BASEL, 2021, 13 (12):
  • [50] Expression of foot-and-mouth disease virus non-structural protein 3A upregulates the expression of autophagy and immune response genes in vitro
    Lalzampuia, H.
    Ganji, Vishweshwar Kumar
    Elango, Subhadra
    Krishnaswamy, Narayanan
    Umapathi, V.
    Reddy, Golla Ramalinga
    Sanyal, Aniket
    Dechamma, H. J.
    VIRUS RESEARCH, 2021, 292
12345