Selectivity and stability of N-terminal targeting protein modification chemistries

被引：4
|
作者：
Barber, Lydia J. [1 ,2 ,3 ]
Yates, Nicholas D. J. [1 ,2 ]
Fascione, Martin A. [1 ,2 ]
Parkin, Alison [1 ]
Hemsworth, Glyn R. [4 ,5 ]
Genever, Paul G. [2 ,3 ]
Spicer, Christopher D. [1 ,2 ,3 ]
机构：
[1] Univ York, Dept Chem, Heslington YO10 5DD, England
[2] Univ York, York Biomed Res Inst, Heslington YO10 5DD, England
[3] Univ York, Dept Biol, Heslington YO10 5DD, England
[4] Univ Leeds, Fac Biol Sci, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, England
[5] Univ Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Leeds LS2 9JT, England
来源：
RSC CHEMICAL BIOLOGY | 2023年 / 4卷 / 01期
基金：
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词：
PEPTIDES;
D O I：
10.1039/d2cb00203e
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein N-termini provide uniquely reactive motifs for single site protein modification. Though a number of reactions have been developed to target this site, the selectivity, generality, and stability of the conjugates formed has not been studied. We have therefore undertaken a comprehensive comparative study of the most promising methods for N-terminal protein modification, and find that there is no 'one size fits all' approach, necessitating reagent screening for a particular protein or application. Moreover, we observed limited stability in all cases, leading to a need for continued innovation and development in the bioconjugation field.
引用
收藏
页码：56 / 64
页数：9
相关论文
共 50 条
  • [21] Axonal targeting of the presynaptic cytomatrix protein Bassoon involves N-terminal sequences
    Dresbach, T
    Zuschratter, W
    Hempelmann, A
    Garner, CC
    Gundelfinger, ED
    EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 : 349 - 349
  • [22] The N-terminal cysteine cluster is essential for membrane targeting of B/K protein
    Fukuda, M
    Mikoshiba, K
    BIOCHEMICAL JOURNAL, 2001, 360 (02) : 441 - 448
  • [23] In silico identification of inhibitors targeting N-Terminal domain of human Replication Protein A
    Cinaroglu, Suleyman Selim
    Timucin, Emel
    JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 2019, 86 : 149 - 159
  • [24] Forespore Targeting of SpoVD in Bacillus subtilis Is Mediated by the N-Terminal Part of the Protein
    Sidarta, Margareth
    Li, Dongdong
    Hederstedt, Lars
    Bukowska-Faniband, Ewa
    JOURNAL OF BACTERIOLOGY, 2018, 200 (13)
  • [25] N-terminal modification of proteins for fluorescence measurements
    Wu, PG
    Brand, L
    FLUORESCENCE SPECTROSCOPY, 1997, 278 : 321 - 330
  • [26] N-Terminal Processing and Modification of Ciliary Dyneins
    Sakato-Antoku, Miho
    Balsbaugh, Jeremy L.
    King, Stephen M.
    CELLS, 2023, 12 (20)
  • [27] AgeMTPT, a Catalyst for Peptide N-Terminal Modification
    Cong, Ying
    Scesa, Paul D.
    Schmidt, Eric W.
    ACS SYNTHETIC BIOLOGY, 2022, 11 (11): : 3699 - 3705
  • [28] N-terminal modification of tau involved in neurodegeneration
    Tanaka, Toshihisa
    Terada, Miyuki
    Sato, Masahiro
    Takeda, Masatoshi
    INTERNATIONAL PSYCHOGERIATRICS, 2013, 25 : S81 - S81
  • [29] N-terminal modification of proteins with o -aminophenols
    Francis, M.B. (mbfrancis@berkeley.edu), 1600, American Chemical Society (136):
  • [30] Targeting Intracellular Pathogenic Bacteria Through N-Terminal Modification of Cationic Amphiphilic Polyproline Helices
    Dietsche, Thomas A.
    Eldesouky, Hassan E.
    Zeiders, Samantha M.
    Seleem, Mohamed N.
    Chmielewski, Jean
    JOURNAL OF ORGANIC CHEMISTRY, 2020, 85 (11): : 7468 - 7475
12345