The antibacterial and antibiofilm effect of amoxicillin and Mangifera indica L. leaves extract on oral pathogens

Objective: This study aimed to determine the antibacterial and antibiofilm effects of amoxicillin combined with extract of Mangifera indica L. leaves against Staphylococcus aureus and Porphyromonas gingivalis. Materials and Methods: This was an experimental laboratory in vitro study with a posttest-only control group design. An antibacterial test using the plate count method and an antibiofilm test using the microtiter plate biofilm assay method were conducted. The research samples comprised extract of M. indica L. leaves with a concentration of 100%; amoxicillin and extract concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%; and amoxicillin. Dimethyl sulfoxide served as a negative control and co-amoxiclav served as a positive control. Results: The combination of amoxicillin and the extract exhibited an antibacterial effect against S. aureus at a concentration of 12.5% and higher and more effective than co-amoxiclac P. gingivalis at a concentration of 3.125% and higher. In the antibiofilm test, the combination of amoxicillin and the extract at a concentration of 25% after 1 h of incubation and a concentration of 6.25% after 3 h of incubation inhibited S. aureus. The inhibition of S. aureus biofilms at a concentration of 100% after 24 h of incubation was as effective as that of co-amoxiclav. The extract at a concentration of 25% over the entire incubation period showed more potent inhibition against the P. gingivalis biofilm than co-amoxiclav. Conclusions: The ethanolic extract of M. indica L. leaves and the combination of amoxicillin and the extract have the potential to inhibit the growth and formation of S. aureus and P. gingivalis biofilms.