Biofilm Formation by Escherichia coli Isolated from Urinary Tract Infections from Aguascalientes, Mexico

被引:2
|
作者
Castillo, Flor Yazmin Ramirez [1 ]
Barrera, Alma Lilian Guerrero [1 ]
Harel, Josee [2 ]
Gonzalez, Francisco Javier Avelar [3 ]
Vogeleer, Philippe [4 ]
Guerra, Jose Manuel Arreola [5 ]
Gamez, Mario Gonzalez [6 ]
机构
[1] Univ Autonoma Aguascalientes, Ctr Ciencias Bas, Dept Morfol, Lab Biol Celular & Tisular, Aguascalientes 20100, Mexico
[2] Univ Montreal, Fac Medecine Veterinaire, Ctr Rech Infectol Porcine & Avicole, Dept Pathol & Microbiol, St Hyacinthe, PQ J2S 7C6, Canada
[3] Univ Autonoma Aguascalientes, Ctr Ciencias Bas, Dept Fisiol & Farmacol, Lab Estudios Ambientales, Aguascalientes 20100, Mexico
[4] Univ Toulouse, Toulouse Biotechnol Inst, INSA, UPS, F-31077 Toulouse, France
[5] Hosp Centenario Miguel Hidalgo, Dept Nefrol, Aguascalientes 20259, Mexico
[6] Hosp Centenario Miguel Hidalgo, Dept Infectol, Aguascalientes 20259, Mexico
基金
加拿大自然科学与工程研究理事会;
关键词
UPEC; biofilm; virulence factors; antimicrobial resistance; multidrug resistance; ANTIBIOTIC-RESISTANCE; VIRULENCE FACTORS; UROVIRULENCE FACTORS; TYPE-1; FIMBRIAE; STRAINS; EPIDEMIOLOGY; PERSISTENCE; OUTPATIENTS; GENES;
D O I
10.3390/microorganisms11122858
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Uropathogenic Escherichia coli (UPEC) strains are among the leading causes of urinary tract infections (UTIs) worldwide. They can colonize the urinary tract and form biofilms that allow bacteria to survive and persist, causing relapses of infections and life-threatening sequelae. Here, we analyzed biofilm production, antimicrobial susceptibility, virulence factors, and phylogenetic groups in 74 E. coli isolated from diagnosed patients with UTIs to describe their microbiological features and ascertain their relationship with biofilm capabilities. High levels of ceftazidime resistance are present in hospital-acquired UTIs. Isolates of multidrug resistance strains (p = 0.0017) and the yfcV gene (p = 0.0193) were higher in male patients. All the strains tested were able to form biofilms. Significant differences were found among higher optical densities (ODs) and antibiotic resistance to cefazolin (p = 0.0395), ceftazidime (p = 0.0302), and cefepime (p = 0.0420). Overall, the presence of fimH and papC coincided with strong biofilm formation by UPEC. Type 1 fimbriae (p = 0.0349), curli (p = 0.0477), and cellulose (p = 0.0253) production was significantly higher among strong biofilm formation. Our results indicated that high antibiotic resistance may be related to male infections as well as strong and moderate biofilm production. The ability of E. coli strains to produce biofilm is important for controlling urinary tract infections.
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页数:18
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