Investigation of Leishmania RNA Virus 2 in Leishmania major and Leishmania tropica Strains Isolated from Cutaneous Leishmaniasis Patients in Turkiye

被引:0
|
作者
Karabulut, Cankut [1 ]
Aksoy, Tulay [1 ]
Yildirim, Ahmet [1 ]
Balcioglu, I. Cuneyt [1 ]
机构
[1] Manisa Celal Bayar Univ, Dept Med Parasitol, Fac Med, Manisa, Turkiye
来源
MIKROBIYOLOJI BULTENI | 2023年 / 57卷 / 03期
关键词
Leishmania major; Leishmania tropica; Leishmania RNA Virus; LRV2; SEQUENCE;
D O I
10.5578/mb.20239938
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus that is thought to contribute to the severe inflammatory response of the causative Leishmania parasite in the mammalian host by being present in many isolates of Leishmania spp. In our study, it was aimed to obtain data on the presence of Leishmania RNA Virus 2 (LRV2), which is thought to cause a change in the clinical course of leishmaniasis, in Leishmania major and Leishmania tropica isolates isolated from cutaneous leishmaniasis (CL) patients in Turkiye. Leishmania strains stored in liquid nitrogen tank by cryopreservation in Manisa Celal Bayar University Faculty of Medicine Parasite Bank were resuscitated under suitable conditions and cultivated in NNN and RPMI-1640 media. Then, the isolates were allowed to enter the logarithmic phase in a 26 degrees C incubator and DNA isolations were made using the "High Pure PCR Template Preparation Kit". Real-time polymerase chain reaction (Rt-PCR) melting analyzes were applied to the DNAs obtained by using primers and probes specific to the internal transcribed spacer-1 (ITS-1) gene region of Leishmania. After RNA isolation from promastigote suspension, cDNA synthesis was performed by reverse transcription. After gel electrophoresis with PCR amplification products, dsRNA band formation was evaluated in terms of LRV2 positivity under ultraviolet light. Among the 20 examined Leishmania spp. isolates (10 L.tropica and 10 L.major), four (three L.tropica, one L.major) were found to be positive for the presence of LRV2. Although the mechanism of LRV in recent studies has not been fully understood, it is known that it exacerbates the clinic of the disease and even has an effect on the formation of drug resistance by the parasite. It is important to obtain data on the presence of LRV in our country and to contribute to various clinical, drug development, prevalence studies, diagnosis and treatment of the disease in the future.
引用
收藏
页码:463 / 472
页数:10
相关论文
共 50 条
  • [11] Successful Isolation of Leishmania RNA Virus (LRV) from Leishmania major in a Cutaneous Leishmaniasis Focus in Central Iran: An Update on Cases
    Moin-Vaziri, Vahideh
    Zare, Fatemeh
    Seyyed Tabaei, Seyyed Javad
    Saberi, Reza
    Hajjaran, Homa
    [J]. ACTA PARASITOLOGICA, 2022, 67 (03) : 1290 - 1298
  • [12] CUTANEOUS LEISHMANIASIS DUE TO LEISHMANIA-TROPICA IN JORDAN
    SALIBA, E
    SALEH, N
    BISHARAT, Z
    OUMEISH, O
    KHOURY, S
    GRAMICCIA, M
    GRADONI, L
    [J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1993, 87 (06) : 633 - 633
  • [13] Miltefosine to treat cutaneous leishmaniasis caused by Leishmania tropica
    Killingley, B.
    Lamb, L. E. M.
    Davidson, R. N.
    [J]. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2009, 103 (02): : 171 - 175
  • [14] CUTANEOUS LEISHMANIASIS CAUSED BY LEISHMANIA-TROPICA IN KENYA
    MEBRAHTU, Y
    OSTER, CN
    SHATRY, AM
    HENDRICKS, LD
    GITHURE, JI
    REES, PH
    PERKINS, PV
    LEEUWENBURG, J
    [J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1987, 81 (06) : 923 - 924
  • [15] A new focus of cutaneous leishmaniasis caused by Leishmania tropica
    Yaghoobi-Ershadi, MR
    Hanafi-Bojd, AA
    Javadian, E
    Jafari, R
    Zahraei-Ramazani, AR
    Mohebali, M
    [J]. SAUDI MEDICAL JOURNAL, 2002, 23 (03) : 291 - 294
  • [16] Efficacy of Systemic Treatment for Leishmania tropica Cutaneous Leishmaniasis
    Solomon, Michal
    Greenberger, Shoshana
    Milner, Maya
    Pavlotzky, Felix
    Barzilai, Aviv
    Schwartz, Eli
    Hadayer, Noa
    Baum, Sharon
    [J]. ACTA DERMATO-VENEREOLOGICA, 2022, 102
  • [17] Concurrent ocular and cutaneous leishmaniasis caused by Leishmania tropica
    Mittal, A.
    Ray, Y.
    Soneja, M.
    Chatterjee, M.
    Kumar, P.
    [J]. QJM-AN INTERNATIONAL JOURNAL OF MEDICINE, 2020, 113 (04) : 286 - 287
  • [18] CHEMOTHERAPY OF CUTANEOUS LEISHMANIASIS - LEISHMANIA TROPICA INFECTIONS IN MICE
    NEAL, RA
    [J]. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1964, 58 (04): : 420 - &
  • [19] Cutaneous and visceral tropisms of Leishmania tropica/Leishmania infantum hybrids in a murine model: First report of hybrid Leishmania strains isolated in Turkey
    Ozbilgin, A.
    Culha, G.
    Zeyrek, F. Yildiz
    Toz, S.
    Gunduz, C.
    Kurt, O.
    Pratlong, F.
    Ozbel, Y.
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2012, 16 : E165 - E165
  • [20] Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
    Kleschenko, Yuliya
    Grybchuk, Danyil
    Matveeva, Nadezhda S.
    Macedo, Diego H.
    Ponirovsky, Evgeny N.
    Lukashev, Alexander N.
    Yurchenko, Vyacheslav
    [J]. GENES, 2019, 10 (10)