Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute

被引：11
|
作者：
Zhao, Yanli [1 ,2 ]
Zhang, Yuhua [1 ,2 ]
Wu, Weiqing [1 ,2 ]
Kang, Tianhao [1 ,2 ]
Sun, Jian [1 ,2 ]
Jiang, Hongxia [1 ,2 ]
机构：
[1] South China Agr Univ, Coll Vet Med, Guangdong Key Lab Vet Pharmaceut Dev & Safety eval, Guangzhou 510642, Peoples R China
[2] South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
来源：
POULTRY SCIENCE | 2024年 / 103卷 / 03期
关键词：
Mycoplasma synoviae; Pyrococcus furiosus Argonaute; recombinase polymerase; detection; AMPLIFICATION; PCR; GALLISEPTICUM; POULTRY; ASSAY;
D O I：
10.1016/j.psj.2023.103244
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
amplification and detection procedures can be completed within 40 min, whereas the results were superficially interpreted under UV and blue light. The sensitivity for MS detection was 2 copies/mL, and the specificity results showed no cross reaction with other pathogens. For the detection of 31 clinical samples, the results of this method and qPCR were completely consistent. This method provides a reliable and convenient method for the on-site detection of MS that is easy to operate without complex instruments and equipment. amplification and detection procedures can be completed within 40 min, whereas the results were superficially interpreted under UV and blue light. The sensitivity for MS detection was 2 copies/mL, and the specificity results showed no cross reaction with other pathogens. For the detection of 31 clinical samples, the results of this method and qPCR were completely consistent. This method provides a reliable and convenient method for the on-site detection of MS that is easy to operate without complex instruments and equipment.
引用
收藏
页数：9
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