Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

被引:1
|
作者
Chileshe, Chikwanda [1 ]
Shawa, Misheck [2 ]
Phiri, Nelson [3 ]
Ndebe, Joseph [4 ]
Khumalo, Cynthia Sipho [1 ]
Nakajima, Chie [5 ]
Kajihara, Masahiro [2 ,6 ]
Higashi, Hideaki [7 ]
Sawa, Hirofumi [2 ,6 ,8 ,9 ]
Suzuki, Yasuhiko [5 ,6 ,9 ]
Muleya, Walter [1 ]
Hang'ombe, Bernard Mudenda [10 ,11 ]
机构
[1] Univ Zambia, Sch Vet Med, Dept Biomed Sci, Lusaka 10101, Zambia
[2] Univ Zambia, Hokudai Ctr Zoonosis Control Zambia, Lusaka 10101, Zambia
[3] Dept Med Control, Zambia Med Regulatory Author, Lusaka 10101, Zambia
[4] Univ Zambia, Sch Vet Med, Dept Dis Control, Lusaka 10101, Zambia
[5] Hokkaido Univ, Div Bioresources, Int Inst Zoonosis Control, N20 W10,Kita ku, Sapporo 0010020, Japan
[6] Hokkaido Univ, Int Inst Zoonosis Control, Div Int Res Promot, N20 W10,Kita ku, Sapporo 0010020, Japan
[7] Hokkaido Univ, Int Inst Zoonosis Control, Div Infect & Immun, N20 W10,Kita ku, Sapporo 0010020, Japan
[8] Hokkaido Univ, Inst Vaccine Res & Dev HU IVReD, N21 W11,Kita ku, Sapporo 0010020, Japan
[9] Hokkaido Univ, Int Inst Zoonosis Control, Div Mol Pathobiol, N20 W10,Kita ku, Sapporo 0010020, Japan
[10] Univ Zambia, Sch Vet Med, Dept Para Clin Studies, Lusaka 10101, Zambia
[11] Univ Zambia, Africa Ctr Excellence Infect Dis Humans & Anim, Sch Vet Med, Lusaka 10101, Zambia
来源
ANTIBIOTICS-BASEL | 2024年 / 13卷 / 03期
关键词
Enterobacteriaceae; bla (CTX-M); bla (TEM); RESISTANCE; POULTRY; GENES; PREVALENCE;
D O I
10.3390/antibiotics13030259
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Poultry products in Zambia form an integral part of the human diet in many households, as they are cheap and easy to produce. The burden of poultry diseases has, however, remained a major challenge. Growing consumer demand for poultry products in Zambia has resulted in non-prudent antimicrobial use on farms, intending to prevent and treat poultry diseases for growth optimisation and maximising profits. This cross-sectional study aimed to identify the different types of bacteria causing diseases in chickens in Lusaka and to detect the extended-spectrum lactamase (ESBL)-encoding genes. We collected 215 samples from 91 diseased chickens at three post-mortem facilities and screened them for Gram-negative bacteria. Of these samples, 103 tested positive for various clinically relevant Enterobacteriaceae, including Enterobacter (43/103, 41.7%), Escherichia coli (20/103, 19.4%), Salmonella (10/103, 9.7%), and Shigella (8/103, 7.8%). Other isolated bacteria included Yersinia, Morganella, Proteus, and Klebsiella, which accounted for 21.4%. E. coli, Enterobacter, Salmonella, and Shigella were subjected to antimicrobial susceptibility testing. The results revealed that E. coli, Enterobacter, and Shigella were highly resistant to tetracycline, ampicillin, amoxicillin, and trimethoprim-sulfamethoxazole, while Salmonella showed complete susceptibility to all tested antibiotics. The observed resistance patterns correlated with antimicrobial usage estimated from sales data from a large-scale wholesale and retail company. Six (6/14, 42.9%) E. coli isolates tested positive for bla(CTX-M), whilst eight (8/14, 57.1%) Enterobacter samples tested positive for bla(TEM). Interestingly, four (4/6, 66.7%) of the E. coli isolates carrying bla(CTX-M)-positive strains were also positive for bla(TEM). Sanger sequencing of the PCR products revealed that five (5/6, 83.3%) of the abovementioned isolates possessed the bla(CTX-M-15) allele. The results suggest the presence of potentially pathogenic ESBL-producing Enterobacteriaceae in poultry, threatening public health.
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页数:13
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