How to fix DNA breaks: new insights into the mechanism of non-homologous end joining

被引：4

作者：

Vogt, Alex ^{[1
,2
]}

He, Yuan ^{[1
,2
,3
,4
]}

Lees-Miller, Susan P. ^{[5
,6
]}

机构：

[1] Northwestern Univ, Dept Mol Biosci, Evanston, IL USA

[2] Northwestern Univ, Interdisciplinary Biol Sci Program, Evanston, IL USA

[3] Northwestern Univ, Chem Life Proc Inst, Evanston, IL USA

[4] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL USA

[5] Univ Calgary, Dept Biochem & Mol Biol, Robson DNA Sci Ctr, Calgary, AB T2N 4N1, Canada

[6] Univ Calgary, Arnie Charbonneau Canc Inst, Calgary, AB T2N 4N1, Canada

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2023年 / 51卷 / 05期

关键词：

DEPENDENT PROTEIN-KINASE; DOUBLE-STRAND BREAK; CRYO-EM STRUCTURE; CATALYTIC SUBUNIT; CRYSTAL-STRUCTURE; STRUCTURAL INSIGHTS; REPAIR; PKCS; AUTOPHOSPHORYLATION; COMPLEX;

D O I：

10.1042/BST20220741

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-homologous end joining (NHEJ) is the major pathway for the repair of ionizing radiation-induced DNA double-strand breaks (DSBs) in human cells and is essential for the generation of mature T and B cells in the adaptive immune system via the process of V(D) J recombination. Here, we review how recently determined structures shed light on how NHEJ complexes function at DNA DSBs, emphasizing how multiple structures containing the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) may function in NHEJ. Together, these studies provide an explanation for how NHEJ proteins assemble to detect and protect DSB ends, then proceed, through DNA-PKcs-dependent autophosphorylation, to a ligation-competent complex.

引用

页码：1789 / 1800

页数：12

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