Nesterenkonia marinintestina sp. nov., isolated from the fish intestine

A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115(T), was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow-green, circular, smooth, central bulge, convex, opaque and 2.0-3.0 mm in diameter after growth on 2216E medium at 30 degrees C for 72 h. Growth occurred at 4-45 degrees C (optimum 30 degrees C), at pH 4.5-10.0 (optimum pH 7.5) and in the presence of 0-12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115(T) was MK-7. The major cellular fatty acids were anteiso-C-15:0 (44.8%), anteiso-C-17:0 (20.5%), and iso-C-15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was L-Lys-Gly-D-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115(T) is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009(T) (96.5%) and Nesterenkonia lutea YIM 70081(T) (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115(T) were 74.49-74.78%, to the two aforementioned type strains, and the digital DNA-DNA hybridization (dDDH) values were 20.1-20.7%. Strain GX14115(T) was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115(T) (= MCCC 1K06658(T) = KCTC 49495(T)).