Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica

During our studies focused on the microorganism diversity and community structure of Populus euphratica at Ebinur lake wetland nature reserve in Xinjiang Uyghur Autonomous Region, PR China, a Gram-positive, aerobic, short rod-shaped bacterium without flagellum, designated as MD2(T), was isolated from a piece of resina on Populus euphratica. The isolate grew at temperature of 10-45 degrees C (optimum 37 degrees C), pH of 7.0-12.0 (optimum pH 8.0) and NaCl concentration of 1-18% (optimum 3%, w/v). Based on the 16S rRNA gene sequences and the phylogenetic analysis, the strain shared the highest sequence similarities to Nesterenkonia alkaliphila JCM 19766(T) (96.3%), Nesterenkonia populi KCTC 29119(T) (95.9%), Nesterenkonia alba CCTCC AB 207011(T) (95.5%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The draft genome of the isolate was sequenced, which comprised 3,739,891 bp with G + C content of 63 mol%, and was annotated to contain 3614 protein-coding genes, 44 tRNA genes and 5 rRNA genes. Chemotaxonomic analysis indicated that the main respiratory quinones were MK-8 and MK-9, the predominant cellular fatty acids were anteiso-C-17:0, anteiso-C-15:0 and iso-C-16:0, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol andphosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain MD2(T) is considered to represent a novel species, for which the name Nesterenkonia ebinurensis sp. nov. is proposed. The type strain is MD2(T) (= KCTC 52999(T) = MCCC 1K03343(T)).