Isolation, purification and characterization of polyhydroxyalkanotes from green algae (Chlorella sp.)

被引：0
|
作者：
Dhivya, M. [1 ]
Mirunalini, A. [1 ]
Vasan P, Thirumalai [1 ]
机构：
[1] Bharathidasan Univ, Srimad Andavan Arts & Sci Coll Autonomous, PG & Res Dept Biotechnol, Trichy 5, Tamil Nadu, India
来源：
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2023年 / 18卷 / 01期
关键词：
Bio polymer; PHA; Chlorella sp; Media; UV Spectrophotometer; FTIR; VULGARIS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Unlike commercial plastic, biopolymers are made from biomass resources that are renewable. The usage of petrochemical plastics raised environmental concerns and the price of oil. A good alternative source of commercially available plastics is discovered to be the intracellular chemical Poly Hydroxy Alkonates (PHA) identified in Chlorella sp., a green algae. Chlorella sp. was isolated and Sudan black B and Nile blue A stain were used to analyze PHA. Samples from the increased biomass production were cultivated using synthetic, natural and affordable media for the sake of optimizing large-scale production. The PHA was extracted, purified and characterized by UV-visible spectrophotometry for the large-scale synthesis using inexpensive media and natural media in various concentrations such as 50%, 70% and 100%.
引用
收藏
页码：94 / 99
页数：6
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