Isolation, purification and characterization of polyhydroxyalkanotes from green algae (Chlorella sp.)

Unlike commercial plastic, biopolymers are made from biomass resources that are renewable. The usage of petrochemical plastics raised environmental concerns and the price of oil. A good alternative source of commercially available plastics is discovered to be the intracellular chemical Poly Hydroxy Alkonates (PHA) identified in Chlorella sp., a green algae. Chlorella sp. was isolated and Sudan black B and Nile blue A stain were used to analyze PHA. Samples from the increased biomass production were cultivated using synthetic, natural and affordable media for the sake of optimizing large-scale production. The PHA was extracted, purified and characterized by UV-visible spectrophotometry for the large-scale synthesis using inexpensive media and natural media in various concentrations such as 50%, 70% and 100%.